ONCOLOGY

Gene
LHB

Uniprot
P01229

Protein
Lutropin subunit beta

See alternative names
Luteinizing hormone subunit beta

Uniprot Function Description

Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.

Additional notes

This assay is designed to detect the beta-subunit (LHB) of Luteinizing hormone (LH). LHB shares ~85% amino acid sequence identity with CGB3 (P0DN86), a beta-subunit of Chorionic gonadotropin (hCG), and the LHB assay shows cross-reactive signal with CGB3. In some samples there is no contribution at all, while others show 70% signal contribution. Samples with high levels of CGB3 show no increased levels of LHB.

Sample type

Human EDTA plasma and serum are the recommended sample types. Human citrate plasma and heparin plasma are also accepted. For other samples types e.g cerebrospinal fluid, (CSF), tissue or cell lysates please we recommend Olink Target 96 panels. Please note that protein expression levels are expected to vary in different sample types and certain assays may be affected by interfering substances such as hemolysate.

Precision

Precision (repeatability) is calculated from linearized NPX values over LOD.

Within run precision Coefficient of Variation (CV)
10%
Between run precision Coefficient of Variation (CV)
16%

Analytical measuring range

The technical data reported below refers to the measured value in the in vitro validation assays run using known concentrations of recombinant antigen. Please note that these figures are for reference only and CANNOT be used to convert NPX values to absolute concentrations for proteins measured in plasma or serum samples.

LOD (pg/ml)
6250
LLOQ (pg/ml)
12500
ULOQ (pg/ml)
800000
Hook (pg/ml)
1600000
Range (log10)
1.8

Dilution factor

For optimal assay readout, Olink Explore is run using different dilutions of the original samples (undiluted, 1:10, 1:100 or 1:1000). The dilution factor for this assay is noted below and should be taken into account when estimating the appropriate addressable biological concentration of the protein based on the in vitro validation data.

Dilution factor
1:1

Sensitivity plot

The calibrator curve shown below visualizes the analytical measuring range data based on in vitro measurement of recombinant antigen. Please note that this is shown for reference only and CANNOT be used to convert NPX values to absolute concentrations for proteins measured in plasma or serum samples. The vertical dotted lines represent LLOQ and ULOQ respectively, and the horizontal line indicates the LOD.

Sample distribution plot

The plot below shows the levels of protein measured in a number of commercial plasma samples. Healthy subjects are shown in blue and samples obtained from patients with a range of diseases are shown in red. The latter include inflammatory, cardiovascular, autoimmune & neurological diseases, as well as cancer. The data is shown to give a general idea of the sort of NPX range to expect, but cannot cover all potential levels that may be seen in clinical samples.